|
CABRI:ICLC |
|
Accession_number |
ICLC ATL97004
|
|
Cell_line_name |
B3/AN
|
|
Brief_description |
Species: mouse, AKR; Tissue: thymus; Tumor: thymoma
|
|
Description |
Species: mouse, AKR; Tissue: thymus; Tumor: thymoma
|
|
Depositor |
Obtained from ECACC.
|
|
Bibliographic_reference |
J Immunol Methods 1992;154:211 [PMID: 1401954
] |
|
Morphology |
spherical, grown in suspension
|
|
Culture_conditions |
continuous culture; DMEM + 10% HS + 2mM L-Glutamine + 1mM Na pyruvate; Maintain cultures between 3 and 9x105 cells/ml. 37C, 5-10% CO2
|
|
Viruses |
Search for viruses was not performed
|
|
Properties |
Lyt-2/13 negative cell line, studies of the assembly and transport of Lyt-2/13 antigen in the cell surface and definition of the regions of Lyt-2/13 interacting with corresponding antibodies and with MHC Class I molecules
|
|
Release_conditions |
Cell line available for distribution. For non-commercial investigative use only
|
|
Hazard |
-
|
|
Species_validation |
Validated by isoenzymes: confirmed as mouse with MD, NP
|
|
Freezing_medium |
Culture medium + 50% FBS + 10% DMSO
|
|
Sterility |
mycoplasma negative, HOECHST and PCR
|
|
Further_bibliography |
Immunogenetics 1994;40:154 [PMID: 7517914
]
|